Reporter

Part:BBa_K1088006:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-20)


B. subtilis dxs-AmilCP fusion (constitutive promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
    Illegal NgoMIV site found at 1065
    Illegal AgeI site found at 958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1606


Design Notes

We added the linker, because we hoped it would improve the function of the chromoprotein amilCP. However it seems as though this did not work as intended. The promoter and RBS are as strong as we could find them, since we only where interested in knowing if the Dxs where expressed at all.


Source

The parts used in this composite parts derives mainly from E. coli, however the amilCP derives from Acropora millepora.

References